NADPPH-Specific Flavoprotein in Rat Liver Microsomal Fractions
نویسندگان
چکیده
Inrat liver microsomal fractions oxidation of adrenaline was effected by superoxide anion radicals (A), whereas cytochrome c, 2,6-dichlorophenol-indophenol and ferricyanide accepted electrons from NADPH-specific filavoprotein only directly. Nitro Blue Tetrazolium was reduced both by 02and by tbe direct acceptance of electrons. Elevation ofpH and addition of menadione shift the Nitro Blue Tetrazolium reduction towards the O2-dependent pathway. From the values of the kinetic constants for interaction ofadrenaline and Nitro Blue Tetrazolium with NADPH-specific flavoprotein, the rates of generation of 02in rat liver microsomal fraction were determined.
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